Although each institution should evaluate BIs for their own use independently, the data indicate that Attest #1261 monitored the three-minute flash cycles more satisfactorily than the other BIs tested. No survivors were detected after the three-minute exposure. The Attest had 100% survival at zero and one-minute exposures and 94% survival after the two-minute exposure. Tec Test was not sufficiently resistant as survivors were not detected at any exposure time. Consists of a Bacillus stearothermophilus spore strip, sealed glass ampule. Proof Flash presented technical difficulties due to incomplete or impossible crushing of media vials, unexpected media color changes, and evaporation of media. Testing, Waste Water, Cosmetics, Consumer Goods, Agriculture and more. Positive BIs were subcultured as necessary. Each cycle was replicated three times on two separate days with six of each BI per run. Survival after "come-up" time alone, (0 exposure) and one-, two-, and three-minute exposures at 273 degrees F in a gravity displacement sterilizer was measured by media color change or turbidity after incubation at 55 degrees C. ![]() ![]() The BIs tested included: 3M's Attest #1261, Amsco's Proof Flash, and Castle Tec Test. ![]() An evaluation of two flash-sterilization-specific biological indicators (BI) and a traditional spore strip indicator was performed to assess sensitivity and reliability as reflected in survive/kill ratios. 3M Attest Rapid Readout Biological Indicator Test Pack, 1296, for 250F/121C Gravity and 270F/132C Vacuum Assisted S.
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